HepG2 cells were subjected to SDS PAGE followed by western blot with FNab03567( GOT1 Antibody) at dilution of 1:1000
IP Result of anti-GOT1 (IP: FNab03567, 4ug; Detection: FNab03567 1:1000) with mouse brain tissue lysate 3600ug.
Background
Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase(3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.